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| WBPicture0000014380 | Description | Fig 2. Intestinal ILYS-3::mCherry associates with endocytic and recycling endosomes and LROs.(A-C) Confocal images of the middle focal plane of the intestine showing that ILYS-3::mCherry partially colocalizes with a subpopulation of GFP::RAB-7 in the median cytoplasm in late endosomes, labeled by both ILYS-3::mCherry (A) and GFP:RAB-7 (B). (C) Overlay of the corresponding red and green channel, magnified 3x region in inset. (D-F) ILYS-3::mCherry occasionally appears in RAB-11 positive vesicles residing in the middle focal plane of the intestine. No double labeling is seen on the apical side of the intestine. (D) ILYS-3::mCherry. (E) GFP::RAB-11. (F) Overlay of the corresponding red and green channel, magnified 3x region in inset. (G-I) ILYS-3::mCherry colocalizes with LysoTracker Green in the intestinal lysosome-related organelles (LROs). (G) ILYS-3::mCherry. (H) LysoTracker Green is internalized apically by the intestinal cells and accumulates in the acidic LROs (green granules). Inset in (I) shows the doubly marked LROs. No red signal is seen in the apical side of the intestine. (J-K) Images of the top focal plane showing the basolateral compartment of the intestine. (J) Micrograph acquired underneath the basolateral membrane showing a population of ILYS-3::mCherry positive vesicles in the cytosol in a wild-type control. (K) ILYS-3::mCherry accumulates in the recycling endosome tubular network in the rme-1 mutant. (L) ILYS-3::mCherry accumulates apically in the plasma membrane and in puncta in rab-10 mutants. Micrograph acquired in themiddle focal plane of the intestine. Asterisks depicts the lumen of the intestine. Arrows point to the apical membrane. Arrowheads point to intestinal vesicles. Dashed arrow marks basolateral tubular networkmCherry-labeled. (M) Pearson's correlation coefficient for colocalization of ILYS-3::mCherry signal with GFP::RAB-7, GFP::RAB-11 and LysoTracker Green. Confocal images were from deconvolved 3D stacks acquired in living adults expressing mCherry- and GFP-tagged proteins in the intestinal epithelial cells. Autofluorescence was corrected using Leica LAS X core software. Values for each group represent n = 17/21 areas from 8 animals. Error bars represent SEM. | |||
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| Name | Fig2.jpg | ||||
| Depict | Expr_pattern | Expr13080 | |||
| Cellular_component | GO:0005764 | ||||
| GO:0005770 | |||||
| GO:0044840 | |||||
| GO:0055037 | |||||
| Acknowledgment | Template | Reprinted from <Journal_URL>, <Article_URL>. <Publisher_URL> <Publication_year>. | |||
| Publication_year | 2016 | ||||
| Article_URL | DOI | id | 10.1371/journal.ppat.1005826 | ||
| Journal_URL | PLoSPathogens | ||||
| Publisher_URL | PLoS | ||||
| Reference | WBPaper00049981 |
