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| WBPicture0000014445 | Description | Figure 1 The C. elegans Nogo-A/RTN4 homolog RET-1 is expressed in the nervous system. (A) Alignment of the C-terminus of C. elegans RET-1 with the Nogo-A/RTN4 homologs of mouse, rat and human. The RHD is highlighted in gray. Alignment was generated using ClustalW. (B) The ret-1 locus comprises eight isoforms encoding predicted proteins of 204-3303 amino acids. All isoforms harbor an RHD in their C-termini. The deletion alleles (marked by red bars) used in this study (gk242 and tm390) both affect the RHD and produce frameshifts. The DNA sequence that encodes the RHD is marked by a black bar at the C terminus. We used ret-1 isoform g.2 as a model to study the ret-1 expression pattern and function. The ret-1 isoform g.2 5000 bp promoter used to report expression (ret-1isoformg.2prom::gfp-now written as ret-1prom::gfp) is shown below the locus. (C-G) Expression of the transcriptional ret-1prom::gfp reporter during embryonic and young adult stages. (C-F) At the bean stage, ret-1prom::gfp is diffusely expressed (D); however, robust expression is observed in the twofold stage of the embryo in two head neurons (F). (C, E) DIC images of the fluorescent images shownin (D) and (F). Postembryonically, ret-1prom::gfp is highly expressed in embryonic motor neurons [eMNs, white arrowheads in (G) and (H)] andhermaphrodite-specific neurons [HSNs, red arrowheads in (G)], the head [red arrowhead in (H) points to the nerve ring], and the tail. All images arelateral views, except the ventral view in (G). Anterior to the left. Bars, 10 um. | |||
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| Name | FigA.jpg | ||||
| Depict | Expr_pattern | Expr13182 | |||
| Anatomy | WBbt:0004017 | ||||
| WBbt:0004757 | |||||
| WBbt:0004758 | |||||
| WBbt:0005300 | |||||
| WBbt:0005735 | |||||
| WBbt:0006749 | |||||
| WBbt:0006751 | |||||
| Acknowledgment | Template | WormBase thanks the journal <Journal_URL> for permission to reproduce figures from this article. Please note that this material may be protected by copyright. Reprinted from <Journal_URL>, <Article_URL>. Copyright (<Publication_year>) with permission from <Publisher_URL> | |||
| Publication_year | 2016 | ||||
| Article_URL | DOI | id | 10.1534/genetics.115.185322 | ||
| Journal_URL | Genetics | ||||
| Publisher_URL | GSA | ||||
| Reference | WBPaper00050392 |
