WormBase Tree Display for Picture: WBPicture0000014704

WBPicture0000014704 Description: Figure 2. Dimorphic Transcription of unc-6in the AVG Neuron(A) unc-6 fosmid reporter expression (otIs638;schematically shown in Figure S2) in both sexes atvarious stages. Expression levels were binned into3 categories, normal (bright), dim, and notdetectable (off); for statistical comparison, normaland dim expression were binned together. Cellularsites of expression were identified by characteristic position and morphology of cells (AVG hasan unusually large nucleus), as well as by co-labeling with an AVG-specific landmark reporter(inx-18).(B) Non-dimorphic, broad expression of an unc-40fosmid reporter construct (otIs647; schematicallyshown in Figure S2) in the tail region of the worm(where the PHA>AVG synapse is generated; seeFigure 1A). Expression in early stages shows noobvious differences (not shown). The red arrowsindicate PHB neurons (identified by dye filling) inwhich UNC-40 is expected to function.(C) smFISH showing unc-6 expression at differentlarval stages and, as control for probe specificity,in unc-6 (ev400) mutant animals, which due to apremature stop codon and nonsense-mediatedmRNA decay are not expected to contain unc-6mRNA. For the beeswarm plot quantification, eachdot represents a single animal with the number ofindividual mRNA molecules in AVG indicated.Comparisons in (A) and (C) were done with theWilcoxon rank-sum test (**p < 0.01; ***p < 0.001).
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  Depict: Expr_pattern: Expr13722
      Expr13723
      Expr13724
    Anatomy: WBbt:0003850
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      WBbt:0003870
      WBbt:0003934
      WBbt:0003936
      WBbt:0007849
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  Acknowledgment: Template: Reprinted from <Journal_URL>, <Article_URL>, Copyright <Publication_year>, with permission from <Publisher_URL>.
    Publication_year: 2018
    Article_URL: DOI id 10.1016/j.cub.2018.01.002
    Journal_URL: CurrentBiology
    Publisher_URL: Elsevier
  Reference: WBPaper00053702