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| WBPicture0000014914 | Description | Figure 1 Generation of IYR001 strain containing sas-6(luv1[sas-6::ha]), and its localization in the early C. elegans embryo A) Left panel: Cartoon depicting nucleotide sequences flanking the ha-tag sequence in the designed repair template to generate the sas-6::ha Red text- Silent mutations introduced in the repair template; Teal highlight- part of homology arms. Right panel: Cartoon depicting DNA fragment sizes expected upon PCR followed by NdeI digestion for wild-type and sas-6::ha edited worms. B) Agarose gel electrophoresis of NdeI digested sas-6 PCR products to screen for homozygous edited sas-6(luv1[sas-6::ha]) worms. L: 1Kb DNA ladder, Yellow stars: sas-6::ha homozygous worms. C) Immunostaining with an anti-HA antibody shows localization of SAS-6::HA to the centrosomes (insets) and in the cytoplasm in 1-cell C. elegans embryos during different stages of mitosis. Microtubules (red), SAS-6::HA (green) and DNA (blue). | |||
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| Name | micropub.biology.000141.jpg | ||||
| Depict | Expr_pattern | Expr14334 | |||
| Cellular_component | GO:0005737 | ||||
| GO:0005813 | |||||
| Life_stage | WBls:0000006 | ||||
| Acknowledgment | Template | Reprinted from <Journal_URL> | |||
| Publication_year | 2019 | ||||
| Article_URL | DOI | id | 10.17912/micropub.biology.000141 | ||
| Journal_URL | microPublicationBiology | ||||
| Publisher_URL | Micropublication | ||||
| Reference | WBPaper00057082 |
