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WBPicture0000014926DescriptionFigure 1: FLAG antibody-stained P. pacificus worms using the HIAR-added protocol, as described in the text. Details on generating CRISPR/Cas9-mediated FLAG-epitope tagged alleles, and precise sites and developmental time courses of nuclear expression will be reported elsewhere. (A) Three Ppa-unc-3::2xFLAG larval worms (RS3506) stained with mouse anti-FLAG monoclonal antibody plus Alexa-594-conjugated anti-mouse secondary. (B) Staining of Ppa-unc-86::1xFLAG strain (RS3509) with anti-FLAG two tails (top), one head (bottom) of larve; antibodies as in A.
Nameloer2019.jpg
DepictExpr_patternExpr14477
Expr14478
AcknowledgmentTemplateReprinted from <Journal_URL>
Publication_year2019
Article_URLDOIid10.17912/micropub.biology.000135
Journal_URLmicroPublicationBiology
Publisher_URLMicropublication
ReferenceWBPaper00057228