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WormBase Tree Display for RNAi: WBRNAi00077683

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Name Class

WBRNAi00077683HomolHomol_homolF30H5:RNAi
Sequence_infoDNA_textccaactacgattctcttctgacactcaccaacttggcaagcgtcagtgattcgattcgtggacgaattttgaaagagaaggcaattccaaagattgaggaattctggtttatgacggatcacgagcatttgagagccgccgccgctgagcttcttctcaatttgctctttttcgagaagttttatgaggaaactgttgcgcccggaaccgatcgtctgaaactctgggttctctactcggcagaagttgaagaggaacgtctatcacgtgcctcggcggcaggtttcgccattcttacagaggacgagaacgcttgtgctaggattatggacgagattaaatcatggcctgaggtgttcaaagacattgcaatgcacgaggatgcggaaacccaacgtcgtggccttatgggaattgcgaatataatgcactcgagcaacaaactgtgctccgaaattgtatcgtccgaagtgttccgtgtcctggtcgccgtcacaaagctcggcactatcaatcaggaacgggcaggctcgacggaacaggcgaaacgtggattggaggccgccgagaagtttggactgattaaggcgacggatcgagagatttatgaacgcgaaaatcaaatgagcaccattcaggaataa
ExperimentLaboratoryDP
Date21 Nov 2007 00:00:00
GenotypeNMY-2::GFP
Delivered_byInjection
InhibitsPredicted_geneF30H5.1Inferred_automaticallyRNAi_primary
GeneWBGene00006781Inferred_automaticallyRNAi_primary
TranscriptF30H5.1.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00031424
PhenotypeWBPhenotype:0000436RemarkNMY-2::GFP did not gather into a contractile anterior domain, instead myosin remained at the cortex and often smaller non-contractile punctae were localized throughout the embryo.
WBPhenotype:0000777Remarkoften fails
WBPhenotype:0001044RemarkReduced cortical granual flow and cortical ruffling was reduced during pronuclear migration.
WBPhenotype:0001078Remarkoften fails
WBPhenotype:0001129RemarkCleavage furrow found at a more central location occurring at 48 percent as opposed to the wild type placement of 42 percent of egg length.
WBPhenotype:0001155RemarkFailed polarity establishment as pronuclei meet centrally.
WBPhenotype:0001168
RemarkExact sequence used for RNAi not stated by authors, 644 bp C-termianal portion of spliced coding region sequence used for curation
MethodRNAi