WormBase Tree Display for RNAi: WBRNAi00084451
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| WBRNAi00084451 | Homol | Homol_homol | K02B12:RNAi | ||
|---|---|---|---|---|---|
| T07A9:RNAi | |||||
| Sequence_info | DNA_text | atgagcgcagatgagccgtctccagaagacgaaaaatatctggaaagtctacgagatttgctgaaaatcagccaagaattcgatgcaagtaacgcaaaacagaatgacgaaccagaaaaaacagcagttgaagtggaatcggctgaaaccagaaccgacgaatctgaaaaatcaatcgatattccacgtgaacagcaacttttaccctccgaacgtgttgagcctttaaaatcaatggtggaaccagaatacgtgaaaaaagtgataaggcaaatggatacaatgactgctgaacagctaaaacaagcgttgatgaagattaaagtgtccacaggcggaaacaagaaaactcttcgaaaacgggttgcacaatattatcgaaaggaaaatgcattattgaatcgaaaaatggagccgaatgccgataaaactgcacgcttttttgactatttaattgcaattgatttcgagtgtacgtgcgtcgagattatatacgattacccacacgaaatcatcgaattaccggcagttttgatcgacgttcgggaaatgaaaattatcagcgagtttcgaacctatgtccgacctgtcagaaatccgaagctcagcgaattttgtatgcagtttaccaaaattgcccaagaaacagtcgatgctgctccatatttccgagaggctcttcaacggctgtacacatggatgcgaaaattcaatctcggacagaaaaattccagattcgcatttgttactgatggacctcacgatatgtggaaattcatgcaatttcaatgccttttatcgaatattcgaatgccccatatgttccgaagttttatcaacatcaaaaagacattcaaagagaaattcaatggacttatcaagggaaatggaaaatccggaattgaaaacatgttagaacgtctggatctgtcgtttgttggaaataaacatagtggattggatgatgcaacgaatattgcagcgattgcgattcaaatgatgaagttgaaaattgagttgagaattaatcagaaatgctcgtataaggagaatcaacggagtgctgcgcgaaaggatgaagagcgagagttagaagacgctgcaaacgtggatcttacatcagtcgatatctctcgtcgtgattttcaactttggatgcgtcgtttaccactgaaactgtcctctgtgacacgacgagagttcataaatgaagagtatttggattgtgattcatgtgatgatttgactgatgataaggtgaaacatctacattcttgtgatatttatgaaattttcgatgaaaaaacttcagctagttttacagattcaaagtgtttgatttgctag | |||
| atgctcataccttcgtcgtcatccataccgtcttctttatctgcttcagcatccgattctgagccatcatcactgaacggaagtggtataagtgatcagaatattctacctaattatcatcttcatcatcatctaattaacgagaacgagatggaggcagccaattatgcacaggttatcaaaccaacatgcgaggcattccaagattggccgcatactccaatgctgtatcaacaacctcaacttcatttcatgcttccacaacacgattgggcatatccacatcttgctcaatctctacctcctccacatcatctaacaccgtccactgcagcagttgctgctgcaacaattgcctcacaaagttcaataatcaatcaaacgtctgtggttacaagtacaccttcgtgccaaatcaaacaggaagttgaacgacctgaaattattcagaggttaatgcctccatggccaccagcttatcaattttcttgtgatgatagtggatcagtgagtggagctggtggaccacatcagccattatctgatatttccgatgatagtgaacagacgtgtccagatgatttggaaggatttgcaaaacaatttaagcagagaagaatcaaattaggatacacacaagcagatgtaggtgtggctctcggaacactctacggaaatatattttcccagacaacaatttgtcgttttgaagcgcttcaactctctttcaagaatatgtgcaaactaaagccacttctattcaaatggctcgaggaagctgattcaacgactggctcgcccaattctacatttgaaaaaatgacagggcaagctggaagaaagagaaagaagagaacaagcattgaggtcaatgtaaaatctcgtcttgaattccatttccagtcgaatcagaagccaaatgcacaagagattacacaagttgccatggagttgcagcttgagaaagaggttgtccgtgtctggttctgcaatcgtcgtcaaaaagagaagcgaatagctccaaatcaatacgatgctccacatccaatggctttaaacaatggatatccaatgactgctgaccttttcccatatcaagccgtcgtcaatcattatactcaacagagcccgagacaacaatag | |||||
| Experiment | Laboratory | CM | |||
| Date | 23 Oct 2009 00:00:00 | ||||
| Strain | WBStrain00002259 | ||||
| Treatment | Injected nematodes were transferred to plates to recover for 24 h before being transferred to fresh plates to lay offspring for 24 h. Thirty F1 progenies (L2 stage) were scored at 48 h post injection for the presence or absence of GFP transgene expression. Additionally, we scored a minimum of 30 F1 progenies from three sister plates of nematodes carrying each transgene at 72 h post injection for developmental delays and lethality to assess the efficiency of the ceh-6 knock down. | ||||
| Delivered_by | Injection | ||||
| Inhibits | Predicted_gene | T07A9.5c | Inferred_automatically | RNAi_primary | |
| T07A9.5a | Inferred_automatically | RNAi_primary | |||
| K02B12.1 | Inferred_automatically | RNAi_primary | |||
| T07A9.5b | Inferred_automatically | RNAi_primary | |||
| T07A9.5d | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00001332 | Inferred_automatically | RNAi_primary | ||
| WBGene00000431 | Inferred_automatically | RNAi_primary | |||
| Transcript | T07A9.5b.1 | Inferred_automatically | RNAi_primary | ||
| K02B12.1.1 | Inferred_automatically | RNAi_primary | |||
| T07A9.5d.1 | Inferred_automatically | RNAi_primary | |||
| T07A9.5c.1 | Inferred_automatically | RNAi_primary | |||
| T07A9.5a.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00035475 | ||||
| Phenotype | WBPhenotype:0001278 | Remark | The percentage of animals expressing the vha-13::gfp transgene in the excretory cell was significantly reduced | ||
| Remark | Exact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation. | ||||
| Method | RNAi |
