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WormBase Tree Display for RNAi: WBRNAi00084826

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Name Class

WBRNAi00084826HomolHomol_homolW09C2:RNAi
Sequence_infoDNA_textatgcattatcgtgatgctaactactctatatcccgaaataaagtgaatctacatcatgaagagtttatgttagatggagttgtcggtggcgaggaccatgatatgcagaacacgaatgaagtgcgtgcggagttggactctttgctccgtcttgatcctgacacaaactccatcattgatgctcttcatatttcacaaccaattgagcaggaaaacatggactacgaaggaaaacccgttgaatttactctcggcacttcttctggaggcgcatcactggcaccaacaagctcaacgacggctgcatccattgcaccattttcatacaatacatcagcaaccaactattacaacaccacaccgagtagttatccaatgttcctcaactatcaatatgctggaggcacaacagtgacaactgatatggatgcatttagtggaatggatatgtcaatgaataacggagtttttggaacacaaaacaatccgagttacttctatccaacgacacaactcaacacatatggatatgatacactcgccgctgccacgacagcatctggaattactgttaataacaatcaattgaatgttaatattgtacaaggaaatggaactatcgtaccacagccaatcactcaaaacatcatctccactgtttcaaatgttcaatcatcggttcctatcaataacagtcaacctctcaccccaactggattggctggctgctcgacgtcttcaggatcatcatcggcttcaagctcatcagcaaactcaacttctacaccgaagaacaccatctccaaagcaaaccgatcatcaggaggagccaacaattctcaattcagcacggaagatcgtgagtgtgtcaattgtggagttcacaatactccattatggcgtcgggatggatcaggaaactacctctgcaacgcatgcggcctctacttcaagatgaaccatcatgctcgtccattggtgaagccgaaaaagagacagcagaacgctcaaaaacggaccggaatcgagtgtgtcaactgtcgtacaaatacaacaacactctggagaagaaatggagaagggcatccagtgtgcaatgcttgtggactctactttaaacttcataaggtggaacgtccaatcaccatgaagaaggatggaattcagacccgtaatcgcaagctctccgcaaagggatcgcgaagaatgaagaaagagaatggcggaactccgacttcaatgggaatgcctacaacatcttcaagtatttcatctggaatcgaacttgatcaaagtggagtttggggaatgaaaaacactcaaccaatgctcatgacaccgacagcatatgcattcccagcatccaatttctacttcaactcgatcgaggatcaattggagtataaaacgtgccctccaatgatggtggactttggaggacaaatgaaaaatttgaatttgaactga
ExperimentLaboratoryRV
Date30 Jun 2009 00:00:00
TreatmentTo bypass the embryonic requirement for elt-1, untreated embryos were hatched in the absence of food to obtain L1 larvae, then transferred to RNAi feeding plates. L4 larvae were picked onto individual fresh RNAi feeding plates to assess self-fertility.
Delivered_byBacterial_feeding
InhibitsPredicted_geneW09C2.1aInferred_automaticallyRNAi_primary
W09C2.1bInferred_automaticallyRNAi_primary
W09C2.1dInferred_automaticallyRNAi_primary
W09C2.1fInferred_automaticallyRNAi_primary
W09C2.1cInferred_automaticallyRNAi_primary
W09C2.1eInferred_automaticallyRNAi_primary
GeneWBGene00001249Inferred_automaticallyRNAi_primary
TranscriptW09C2.1e.1Inferred_automaticallyRNAi_primary
W09C2.1f.1Inferred_automaticallyRNAi_primary
W09C2.1c.1Inferred_automaticallyRNAi_primary
W09C2.1a.1Inferred_automaticallyRNAi_primary
W09C2.1d.1Inferred_automaticallyRNAi_primary
W09C2.1b.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00034714
PhenotypeWBPhenotype:0000038RemarkRuptured vulva phenotype observed in the majority (greater than 95%) of treated hermaphrodites.
WBPhenotype:0000154RemarkScored non-ruptured animals.
WBPhenotype:0000357RemarkScored non-ruptured animals.
WBPhenotype:0000387RemarkThe pseudopod of sperm from elt-1 RNAi-treated animals is either absent or short and aberrantly shaped and the cells are unable to crawl.
WBPhenotype:0000509RemarkThe pseudopod of sperm from elt-1 RNAi-treated animals is either absent or short and aberrantly shaped and the cells are unable to crawl.
WBPhenotype:0000688RemarkScored non-ruptured animals.
RemarkExact sequence used for RNAi not stated by authors, spliced coding region sequence of gene used for curation (W09C2.1a).
MethodRNAi