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WormBase Tree Display for RNAi: WBRNAi00084964

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Name Class

WBRNAi00084964HomolHomol_homolF09B9:RNAi
Sequence_infoPCR_productsjj_F09B9.2
ExperimentLaboratoryRZ
Date19 Nov 2010 00:00:00
GenotypeotIs138
TreatmentThree L4 larval stage hermaphrodites were placed on each RNAi plate, incubated for 4 days at 20C and allowed to lay a brood. After incubation, young adult progeny were washed off feeding pates with M9 buffer and mounted on 5% agar pads. Approximately twenty adult progeny per RNAi clone were scored for the presence of defective PVD dendritic morphology, defined here as a qualitative increase or decrease in the number of secondary, tertiary or quaternary dendrites.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneF09B9.2aInferred_automaticallyRNAi_primary
F09B9.2bInferred_automaticallyRNAi_primary
GeneWBGene00006839Inferred_automaticallyRNAi_primary
TranscriptF09B9.2b.1Inferred_automaticallyRNAi_primary
F09B9.2a.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00037977
PhenotypeWBPhenotype:0000882RemarkAnimals exhibited a decrease in the number of quaternary branches of the PVD neuronal dendrites
PenetranceRange6576
EQ_annotationsLife_stageWBls:0000041PATO:0000460
RemarkExact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi