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WormBase Tree Display for RNAi: WBRNAi00084993

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Name Class

WBRNAi00084993HomolHomol_homolW02D3:RNAi
Sequence_infoPCR_productsjj_W02D3.3
ExperimentLaboratoryRZ
Date19 Nov 2010 00:00:00
GenotypeotIs138
TreatmentThree L4 larval stage hermaphrodites were placed on each RNAi plate, incubated for 4 days at 20C and allowed to lay a brood. After incubation, young adult progeny were washed off feeding pates with M9 buffer and mounted on 5% agar pads. Approximately twenty adult progeny per RNAi clone were scored for the presence of defective PVD dendritic morphology, defined here as a qualitative increase or decrease in the number of secondary, tertiary or quaternary dendrites.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneW02D3.3Inferred_automaticallyRNAi_primary
GeneWBGene00003170Inferred_automaticallyRNAi_primary
TranscriptW02D3.3.1Inferred_automaticallyRNAi_primary
W02D3.3.3Inferred_automaticallyRNAi_primary
W02D3.3.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00037977
PhenotypeWBPhenotype:0000882RemarkAnimals exhibited a dendrite branching defect in PVD neurons
PenetranceRange5
EQ_annotationsLife_stageWBls:0000041PATO:0000460
RemarkExact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation.
MethodRNAi