WormBase Tree Display for RNAi: WBRNAi00086319
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| WBRNAi00086319 | Homol | Homol_homol | ZK1321:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_ZK1321.2 | |||
| Experiment | Laboratory | LY | |||
| Date | 09 Aug 2006 00:00:00 | ||||
| Genotype | pshl-1::shl-1::W363F (Dominant negative SHL-1) | ||||
| Treatment | Double-stranded RNA (dsRNA) was synthesized byusing standard methods. Cultured myocytes were plated in L-15 control medium or L-15 medium containing 15 micrograms/ml dsRNA final volume. One hour after plating, the dsRNA was diluted to a final concentration of 5 micrograms/ml. Media containing the dsRNA were replaced each day. Electrophysiological experiments were performed 24 days after plating the cells. | ||||
| Delivered_by | Soaking | ||||
| Inhibits | Predicted_gene | ZK1321.2e | Inferred_automatically | RNAi_primary | |
| ZK1321.2b | Inferred_automatically | RNAi_primary | |||
| ZK1321.2a | Inferred_automatically | RNAi_primary | |||
| ZK1321.2f | Inferred_automatically | RNAi_primary | |||
| ZK1321.2d | Inferred_automatically | RNAi_primary | |||
| ZK1321.2c | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00014261 | Inferred_automatically | RNAi_primary | ||
| Transcript | ZK1321.2a.1 | Inferred_automatically | RNAi_primary | ||
| ZK1321.2e.1 | Inferred_automatically | RNAi_primary | |||
| ZK1321.2f.1 | Inferred_automatically | RNAi_primary | |||
| ZK1321.2b.1 | Inferred_automatically | RNAi_primary | |||
| ZK1321.2c.1 | Inferred_automatically | RNAi_primary | |||
| ZK1321.2d.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00028359 | ||||
| Phenotype | WBPhenotype:0001315 | Remark | RNAi of shk-1 in cultured myocytes (carrying a dominant negative form of SHL-1) resulted in the complete removal of all macroscopic outward (electircal) current. | ||
| Remark | Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. | ||||
| Method | RNAi |
