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WormBase Tree Display for RNAi: WBRNAi00086998

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Name Class

WBRNAi00086998HomolHomol_homolF56B3:RNAi
Sequence_infoPCR_productsjj_F56B3.4
ExperimentLaboratoryGR
Date24 Oct 2007 00:00:00
Genotypeeri-1(mg366); let-7(mg279)
TreatmentSynchronized L1 worms of the eri-1(mg366);let-7(mg279) genotype were placed onto 6-well RNAi plates, about 6 worms (for second generation screening) or 100 (for parental generation screening). These RNAi plates were then kept at 20 C and either scored for bursting 3 days later (for parental generation screening) or shifted to 25 C 3 days later and then scored for bursting 2 days after that (for second generation screening).
Delivered_byBacterial_feeding
InhibitsPredicted_geneF56B3.4bInferred_automaticallyRNAi_primary
F56B3.4aInferred_automaticallyRNAi_primary
F56B3.12Inferred_automaticallyRNAi_primary
GeneWBGene00004824Inferred_automaticallyRNAi_primary
WBGene00018935Inferred_automaticallyRNAi_primary
TranscriptF56B3.4b.1Inferred_automaticallyRNAi_primary
F56B3.4a.1Inferred_automaticallyRNAi_primary
F56B3.12.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000501120
ReferenceWBPaper00031224
PhenotypeWBPhenotype:0000038
RemarkExact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.. Figure 1 names the targeted sequence as F56B3.4 and the locus as skr-18 (which has sequence name F56B3.12 as of WS227); used skr-18/F56B3.12 for curation
MethodRNAi