WormBase Tree Display for RNAi: WBRNAi00087994
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| WBRNAi00087994 | Homol | Homol_homol | CHROMOSOME_I:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | DNA_text | CTTCATTCTCGTTTCATGCGAATTCAGAATGAAGGAGCCGGAAAGAGCTCGTGGTGGGTTATTAATCCAGATGCAAAGCCAGGAAGGAATCCACGGCGTACACGTGAACGATCCAATACTATTGAGACGACTACAAAGGCTCAACTCGAAAAATCTCGCCGCGGAGCCAAGAAGAGGATAAAGGAGAGAGCATTGATGGGCTCCCTTCACTCGACACTTAATGGAAATTCGATTGCCGGATCGATTCAAACGATTTCTCACGATTTGTATGATGATGATTCAATGCAAGGAGCATTTGATAACGTTCCATCATCTTTCCGTCCCCGAACTCAATCGAACCTCTCGATTCCTGGATCGTCGTCTCGTGTTTCTCCAGCTATTGGAAGTGATATCTATGATGATCTAGAATTCCCATCATGGGTTGGCGAATCGGTTCCAGCAATTCCAAGTGATATTGTTGATAGAACTGATCAAATGCGTATCGATGCAACTACTCATATTGGTGGAGTTCAGATTAAGCAGGAGTCGAAGCCGATTAAGACGGAACCAATTGCTCCACCACCATCATACCACGAGTTGAACAGTGTCCGTGGATCGTGTGCTCAGAATCCACTTCTTCGAAATCCAATTGTGCCAAGCACTAACTTCAAGCCAATGCCACTACCGGGTGCCTATGGAAACTATCAAAATGGTGGAATAACTCCAATCAATTGGCTATCAACATCCAACTCATCTCCACTGCCTGGAATTCAATCGTGTGGAATTGTAGCTGCACAGCATACTGTCGCTTCTTCATCGGCTCTTCCAATTGATTTGGAAAATCTGACACTTCCCGATCAGCCACTGATGGATACTATGGATGTTGATGCATTGATCAGACA | |||
| Experiment | Laboratory | HT | |||
| Date | 14 May 2010 00:00:00 | ||||
| Genotype | daf-16(mgDf50); daf-2(e1370); daf-16d/f::gfp | ||||
| Treatment | daf-16(mgDf50); daf-2(e1370) mutant worms carrying low-copy daf-16d/f::gfp transgene were grown on daf-16 isoform RNAi for two generations; then worms were mounted and visualized by fluorescence microscopy | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene (11) | ||||
| Gene | WBGene00000912 | Inferred_automatically | RNAi_primary | ||
| Transcript (11) | |||||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00036474 | ||||
| Phenotype | WBPhenotype:0001278 | Remark | daf-16a/b/d/f isoform-specific RNAi resulted in reduced expression of the daf-16d/f::GFP transgene | ||
| Remark | The RNAi construct specifically targets the daf-16a, daf-16b, daf-16d and daf-16f isoforms for knockdown | ||||
| Method | RNAi |
