WormBase Tree Display for RNAi: WBRNAi00088898
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| WBRNAi00088898 | Homol | Homol_homol | ZK270:RNAi | |||
|---|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_ZK270.1 | ||||
| Experiment | Laboratory | ALF | ||||
| Date | 21 Mar 2011 00:00:00 | |||||
| Genotype | daf-2(e1371); spe-9(hc88); fer-15(b26) | |||||
| Treatment | RNAi treatment was performed by feeding animals double-stranded RNA-producing bacteria as described previously except that we used a 4x concentrated RNAi culture to spot plates. After drying, 50-60 eggs isolated by hypochlorite treatment were added to the RNAi plates and kept at 20 degrees Celsius until the worms reached the L4 stage before being transferred to 25 degrees Celsius. This is the nonpermissive temperature for adult fertility and creates a synchronized adult population. | |||||
| Temperature | 25 | |||||
| Delivered_by | Bacterial_feeding | |||||
| Inhibits | Predicted_gene | ZK270.1 | Inferred_automatically | RNAi_primary | ||
| Gene | WBGene00004237 | Inferred_automatically | RNAi_primary | |||
| Transcript | ZK270.1.1 | Inferred_automatically | RNAi_primary | |||
| ZK270.1.2 | Inferred_automatically | RNAi_primary | ||||
| Species | Caenorhabditis elegans | |||||
| Reference | WBPaper00038430 | |||||
| Phenotype_not_observed | WBPhenotype:0000926 | Remark | Muscle morphology, as visualized by phalloidin staining, was normal. | |||
| EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
| Remark | ZK270.1 (ptr-23) RNAi | |||||
| Method | RNAi |
