WormBase Tree Display for RNAi: WBRNAi00088946
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| WBRNAi00088946 | Homol | Homol_homol | C38C3:RNAi | |||
|---|---|---|---|---|---|---|
| Sequence_info | PCR_product | mv_C38C3.f | ||||
| Experiment | Laboratory | ALF | ||||
| Date | 21 Mar 2011 00:00:00 | |||||
| Treatment | RNAi treatment was performed by feeding animals double-stranded RNA-producing bacteria as described previously except that we used a 4x concentrated RNAi culture to spot plates. After drying, 50-60 eggs isolated by hypochlorite treatment were added to the RNAi plates and kept at 20 degrees Celsius until the worms reached the L4 stage before being transferred to 25 degrees Celsius. | |||||
| Temperature | 25 | |||||
| Delivered_by | Bacterial_feeding | |||||
| Inhibits | Predicted_gene | F53E2.2 | Inferred_automatically | RNAi_primary | ||
| C38C3.5 | Inferred_automatically | RNAi_primary | ||||
| Gene | WBGene00302980 | Inferred_automatically | RNAi_primary | |||
| WBGene00006794 | Inferred_automatically | RNAi_primary | ||||
| Transcript | F53E2.2.1 | Inferred_automatically | RNAi_primary | |||
| C38C3.5.1 | Inferred_automatically | RNAi_primary | ||||
| Species | Caenorhabditis elegans | |||||
| Reference | WBPaper00038430 | |||||
| Phenotype_not_observed | WBPhenotype:0001452 | Remark | Authors report that Day 10 RNAi-treated worms were able to respond appropriately to octanol within a 20-second time frame, similar to controls | |||
| EQ_annotations | Life_stage | WBls:0000041 | PATO:0000460 | |||
| Remark | C38C3.5 (unc-60) RNAi | |||||
| Method | RNAi |
