WormBase Tree Display for RNAi: WBRNAi00091022

WBRNAi00091022 Homol: Homol_homol: M117:RNAi
  Sequence_info: PCR_product: sjj_M117.2
  Experiment: Laboratory: MZE
    Date: 19 Apr 2012 00:00:00
    Genotype: unc-119(ed3) III; cbgIs91[pPept-1:pept-1::DsRed;unc-119(+)]; cbgIs98[pPept-1:GFP::rab-11.1;unc-119(+)]
    Treatment: L1 animals were fed dsRNA-expressing bacteria until the young adult stage, at which point they were imaged and scored for phenotypes
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: M117.2b Inferred_automatically: RNAi_primary
      M117.3 Inferred_automatically: RNAi_primary
      M117.2a Inferred_automatically: RNAi_primary
    Gene: WBGene00003920 Inferred_automatically: RNAi_primary
      WBGene00010919 Inferred_automatically: RNAi_primary
    Transcript: M117.2a.1 Inferred_automatically: RNAi_primary
      M117.2a.2 Inferred_automatically: RNAi_primary
      M117.3.1 Inferred_automatically: RNAi_primary
      M117.2a.3 Inferred_automatically: RNAi_primary
      M117.2b.1 Inferred_automatically: RNAi_primary
      M117.2a.4 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Interaction: WBInteraction000519809
  Reference: WBPaper00041129
  Phenotype: WBPhenotype:0000679 Remark: PEPT-1::DsRED accumulated in fuzzy or irregularly shaped structures; PEPT-1::DsRED fluorescence intensity reduced; GFP::RAB-11 recycling endosomes (REs) localise basally; GFP::RAB-11 recycling endosomes (REs) accumulate within the cell in prominent, peripheral clusters at the basolateral domain of enterocytes; accumulations can be of different size, abundance, and sub-cellular localisation; GFP::RAB-11 fluorescence intensity reduced; Autofluorescent lysosome-related organelles (AF/LROs) are enlarged or accumulated; Effects on RAB-11-recycling endosome (RE) morphology or positioning differ from or are more prominent compared to effects on Golgi, early endosomes or late endosomes
    WBPhenotype:0001278 Remark: PEPT-1::DsRED accumulated in fuzzy or irregularly shaped structures; PEPT-1::DsRED fluorescence intensity reduced; GFP::RAB-11 recycling endosomes (REs) localise basally; GFP::RAB-11 recycling endosomes (REs) accumulate within the cell in prominent, peripheral clusters at the basolateral domain of enterocytes; accumulations can be of different size, abundance, and sub-cellular localisation; GFP::RAB-11 fluorescence intensity reduced; Autofluorescent lysosome-related organelles (AF/LROs) are enlarged or accumulated; Effects on RAB-11-recycling endosome (RE) morphology or positioning differ from or are more prominent compared to effects on Golgi, early endosomes or late endosomes
    WBPhenotype:0002094 Remark: PEPT-1::DsRED accumulated in fuzzy or irregularly shaped structures; PEPT-1::DsRED fluorescence intensity reduced; GFP::RAB-11 recycling endosomes (REs) localise basally; GFP::RAB-11 recycling endosomes (REs) accumulate within the cell in prominent, peripheral clusters at the basolateral domain of enterocytes; accumulations can be of different size, abundance, and sub-cellular localisation; GFP::RAB-11 fluorescence intensity reduced; Autofluorescent lysosome-related organelles (AF/LROs) are enlarged or accumulated; Effects on RAB-11-recycling endosome (RE) morphology or positioning differ from or are more prominent compared to effects on Golgi, early endosomes or late endosomes
    WBPhenotype:0002095 Remark: PEPT-1::DsRED accumulated in fuzzy or irregularly shaped structures; PEPT-1::DsRED fluorescence intensity reduced; GFP::RAB-11 recycling endosomes (REs) localise basally; GFP::RAB-11 recycling endosomes (REs) accumulate within the cell in prominent, peripheral clusters at the basolateral domain of enterocytes; accumulations can be of different size, abundance, and sub-cellular localisation; GFP::RAB-11 fluorescence intensity reduced; Autofluorescent lysosome-related organelles (AF/LROs) are enlarged or accumulated; Effects on RAB-11-recycling endosome (RE) morphology or positioning differ from or are more prominent compared to effects on Golgi, early endosomes or late endosomes
    WBPhenotype:0002107 Remark: PEPT-1::DsRED accumulated in fuzzy or irregularly shaped structures; PEPT-1::DsRED fluorescence intensity reduced; GFP::RAB-11 recycling endosomes (REs) localise basally; GFP::RAB-11 recycling endosomes (REs) accumulate within the cell in prominent, peripheral clusters at the basolateral domain of enterocytes; accumulations can be of different size, abundance, and sub-cellular localisation; GFP::RAB-11 fluorescence intensity reduced; Autofluorescent lysosome-related organelles (AF/LROs) are enlarged or accumulated; Effects on RAB-11-recycling endosome (RE) morphology or positioning differ from or are more prominent compared to effects on Golgi, early endosomes or late endosomes
  Remark: (Table S1, S3, Figure 2a, 3f) M117.2/par-5 RNAi
  Method: RNAi