WormBase Tree Display for RNAi: WBRNAi00094373
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WBRNAi00094373 | Homol | Homol_homol | C42D4:RNAi | |
---|---|---|---|---|
Sequence_info | PCR_product | sjj_C42D4.10 | ||
Experiment | Laboratory | HZ | ||
Date | 18 Oct 2011 00:00:00 | |||
Genotype | bpIs70 [gfp::bro-1(PTC)] | |||
Treatment | Five to seven L4 animals carrying a gfp::bro-1(PTC) transgene were plated onto RNAi feeding plates and were grown at 21 degrees Celsius for 3 days. The F1 L3 or L4 larvae were examined for reporter expression. Expression of gfp::bro-1(PTC) was not detected in late larvae fed on control RNAi clone (n > 100). The ratio of animals expressing gfp::bro-1(PTC) was derived from three separate experiments (at least 30 animals were examined for each RNAi clone). The RNAi clones that caused more than 25% of animals expressing gfp were selected for further analysis. | |||
Temperature | 21 | |||
Delivered_by | Bacterial_feeding | |||
Species | Caenorhabditis elegans | |||
Reference | WBPaper00040559 | |||
Phenotype | WBPhenotype:0000508 | Remark | RNAi resulted in increased larval expression of a gfp::bro-1(PTC) transgene carrying a premature termination codon, indicative of defective mRNA surveillance machinery | |
WBPhenotype:0001236 | Remark | RNAi resulted in increased larval expression of a gfp::bro-1(PTC) transgene carrying a premature termination codon, indicative of defective mRNA surveillance machinery | ||
WBPhenotype:0001538 | Remark | RNAi resulted in increased larval expression of a gfp::bro-1(PTC) transgene carrying a premature termination codon, indicative of defective mRNA surveillance machinery | ||
Remark | (Table S1) str-219 RNAi | |||
Method | RNAi |