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WormBase Tree Display for RNAi: WBRNAi00095876

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Name Class

WBRNAi00095876HomolHomol_homolC01H6:RNAi
Sequence_infoPCR_productsjj_C01H6.5
ExperimentLaboratoryGR
Date23 Feb 2012 00:00:00
StrainWBStrain00000264
TreatmentFor reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates.
Delivered_byBacterial_feeding
InhibitsPredicted_geneC01H6.5bInferred_automaticallyRNAi_primary
C01H6.5fInferred_automaticallyRNAi_primary
C01H6.5dInferred_automaticallyRNAi_primary
C01H6.5aInferred_automaticallyRNAi_primary
C01H6.5cInferred_automaticallyRNAi_primary
C01H6.5eInferred_automaticallyRNAi_primary
GeneWBGene00003622Inferred_automaticallyRNAi_primary
TranscriptC01H6.5d.1Inferred_automaticallyRNAi_primary
C01H6.5a.1Inferred_automaticallyRNAi_primary
C01H6.5c.1Inferred_automaticallyRNAi_primary
C01H6.5b.1Inferred_automaticallyRNAi_primary
C01H6.5f.1Inferred_automaticallyRNAi_primary
C01H6.5e.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00040984
Phenotype_not_observedWBPhenotype:0001236RemarkRNAi does not result in activation of the irg-1::GFP reporter gene
Remark(Table S2A) nhr-23 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
MethodRNAi