WormBase Tree Display for RNAi: WBRNAi00096275
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| WBRNAi00096275 | Homol | Homol_homol | T05E11:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_T05E11.1 | |||
| Experiment | Laboratory | GR | |||
| Date | 23 Feb 2012 00:00:00 | ||||
| Genotype | agIs26[myo-2::mCherry,clec-60::gfp] | ||||
| Treatment | For reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | T05E11.1 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00004474 | Inferred_automatically | RNAi_primary | ||
| Transcript | T05E11.1.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00040984 | ||||
| Phenotype | WBPhenotype:0001236 | Remark | RNAi results in strong activation of the clec-60::GFP clec-60::GFP reporter gene | ||
| Remark | (Table S2A) rps-5 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation. | ||||
| Method | RNAi |
