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WormBase Tree Display for RNAi: WBRNAi00096324

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Name Class

WBRNAi00096324HomolHomol_homolK02D7:RNAi
Sequence_infoPCR_productsjj_K02D7.3
ExperimentLaboratoryGR
Date23 Feb 2012 00:00:00
StrainWBStrain00004861
TreatmentFor reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates.
Delivered_byBacterial_feeding
InhibitsPredicted_geneF18F11.5Inferred_automaticallyRNAi_primary
K02D7.3bInferred_automaticallyRNAi_primary
K02D7.3aInferred_automaticallyRNAi_primary
GeneWBGene00000675Inferred_automaticallyRNAi_primary
WBGene00017578Inferred_automaticallyRNAi_primary
TranscriptK02D7.3a.1Inferred_automaticallyRNAi_primary
F18F11.5.1Inferred_automaticallyRNAi_primary
K02D7.3b.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00040984
Phenotype_not_observedWBPhenotype:0001236RemarkRNAi does not result in activation of the sod-3::GFP reporter gene
Remark(Table S2B) col-101 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
MethodRNAi