WormBase Tree Display for RNAi: WBRNAi00096414

WBRNAi00096414 Homol: Homol_homol: F08C6:RNAi
  Sequence_info: PCR_product: sjj_F08C6.2
  Experiment: Laboratory: GR
    Date: 23 Feb 2012 00:00:00
    Strain: WBStrain00034065
    Treatment: For reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates.
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F08C6.2b Inferred_automatically: RNAi_primary
      F08C6.2a Inferred_automatically: RNAi_primary
    Gene: WBGene00017241 Inferred_automatically: RNAi_primary
    Transcript: F08C6.2a.1 Inferred_automatically: RNAi_primary
      F08C6.2b.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00040984
  Phenotype: WBPhenotype:0001236 Remark: RNAi results in strong activation of the hsp-4::GFP reporter gene
  Remark: (Table S2B) F08C6.2 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
  Method: RNAi