WormBase Tree Display for RNAi: WBRNAi00096541

WBRNAi00096541 Homol: Homol_homol: Y47D3B:RNAi
  Sequence_info: PCR_product: sjj_Y47D3B.7
  Experiment: Laboratory: GR
    Date: 23 Feb 2012 00:00:00
    Strain: WBStrain00034068
    Treatment: For reporter induction experiments, approximately 30-50 animals were serially aliquoted to 12- or 24-well RNAi plates that had been seeded with 200-300 microliters unconcentrated overnight RNAi cultures and dried 3-4 hr in hood on previous day. RNAi plates were either grown at room temperature (23-24 degrees Celsius) for 2 days or 20 degrees Celsius for 3 days prior to scoring (after confirming reproducibility of results between growth conditions.) Each RNAi clone was tested in duplicate on a given day, and results were reproduced in 2-3 independent replicates.
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: Y47D3B.7 Inferred_automatically: RNAi_primary
    Gene: WBGene00004735 Inferred_automatically: RNAi_primary
    Transcript: Y47D3B.7.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00040984
  Phenotype_not_observed: WBPhenotype:0001236 Remark: RNAi does not result in activation of the hsp-6::GFP reporter gene
  Remark: (Table S2B) sbp-1 RNAi. Exact sequence used for RNAi not stated by authors, Ahringer laboratory clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
  Method: RNAi