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WormBase Tree Display for RNAi: WBRNAi00096924

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Name Class

WBRNAi00096924HomolHomol_homolT20G5:RNAi
Sequence_infoPCR_productsjj_T20G5.2
ExperimentLaboratoryERT
Date14 Feb 2012 00:00:00
StrainWBStrain00000264
TreatmentOvernight cultures of RNAi feeding clones were plated onto 24-well RNAi plates and incubated at 25 degrees Celsius for 1 day. About 100 synchronized L1 stage agIs17[irg-1p::GFP] animals per well were fed on these RNAi plates for two days at 20 degrees Celsius until L4/young adult stage. These animals were then screened for GFP expression.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneT20G5.2Inferred_automaticallyRNAi_primary
GeneWBGene00000833Inferred_automaticallyRNAi_primary
TranscriptT20G5.2.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00041034
PhenotypeWBPhenotype:0000031RemarkRNAi induced expression of the irg-1::GFP transgene and resulted in a slow growth phenotype
WBPhenotype:0001236RemarkRNAi induced expression of the irg-1::GFP transgene and resulted in a slow growth phenotype
Remark(Table S1) cts-1 RNAi. Authors state RNAi clones obtained from the Ahringer and Vidal laboratories, Ahringer clone used for curation.
MethodRNAi