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WormBase Tree Display for RNAi: WBRNAi00096957

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Name Class

WBRNAi00096957HomolHomol_homolC47D12:RNAi
Sequence_infoPCR_productsjj_C47D12.6
ExperimentLaboratoryERT
Date14 Feb 2012 00:00:00
Genotypezip-2(tm4248); agIs17[irg-1p::GFP]
TreatmentOvernight cultures of RNAi feeding clones were plated onto 24-well RNAi plates and incubated at 25 degrees Celsius for 1 day. About 100 synchronized L1 stage zip-2(tm4248);agIs17[irg-1p::GFP] animals per well were fed on these RNAi plates for two days at 20 degrees Celsius until L4/young adult stage. These animals were then screened for GFP expression.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneC47D12.6aInferred_automaticallyRNAi_primary
C47D12.6bInferred_automaticallyRNAi_primary
GeneWBGene00006617Inferred_automaticallyRNAi_primary
TranscriptC47D12.6b.1Inferred_automaticallyRNAi_primary
C47D12.6a.1Inferred_automaticallyRNAi_primary
C47D12.6b.2Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00041034
Phenotype_not_observedWBPhenotype:0001236RemarkThe zip-2(tm4248) mutation suppressed the RNAi-induced expression of the irg-1::GFP transgene
Remark(Table S1) tars-1 RNAi. Authors state RNAi clones obtained from the Ahringer and Vidal laboratories, Ahringer clone used for curation.
MethodRNAi