WormBase Tree Display for RNAi: WBRNAi00096963
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| WBRNAi00096963 | Homol | Homol_homol | F31E3:RNAi | ||
|---|---|---|---|---|---|
| C54D2:RNAi | |||||
| Sequence_info | PCR_product | sjj_R03G5.1 | |||
| Experiment | Laboratory | ERT | |||
| Date | 14 Feb 2012 00:00:00 | ||||
| Genotype | zip-2(tm4248); agIs17[irg-1p::GFP] | ||||
| Treatment | Overnight cultures of RNAi feeding clones were plated onto 24-well RNAi plates and incubated at 25 degrees Celsius for 1 day. About 100 synchronized L1 stage zip-2(tm4248);agIs17[irg-1p::GFP] animals per well were fed on these RNAi plates for two days at 20 degrees Celsius until L4/young adult stage. These animals were then screened for GFP expression. | ||||
| Temperature | 20 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | R03G5.1 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00001169 | Inferred_automatically | RNAi_primary | ||
| Transcript | R03G5.1.2 | Inferred_automatically | RNAi_primary | ||
| R03G5.1.3 | Inferred_automatically | RNAi_primary | |||
| R03G5.1.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00041034 | ||||
| Phenotype_not_observed | WBPhenotype:0001236 | Remark | The zip-2(tm4248) mutation suppressed the RNAi-induced expression of the irg-1::GFP transgene | ||
| Remark | (Table S1) eef-1A.2 RNAi. Authors state RNAi clones obtained from the Ahringer and Vidal laboratories, Ahringer clone used for curation. | ||||
| Method | RNAi |
