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WormBase Tree Display for RNAi: WBRNAi00096983

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Name Class

WBRNAi00096983HomolHomol_homolF45E1:RNAi
Sequence_infoPCR_productsjj_F45E1.6
ExperimentLaboratoryERT
Date14 Feb 2012 00:00:00
Genotypezip-2(tm4248); agIs17[irg-1p::GFP]
TreatmentOvernight cultures of RNAi feeding clones were plated onto 24-well RNAi plates and incubated at 25 degrees Celsius for 1 day. About 100 synchronized L1 stage zip-2(tm4248);agIs17[irg-1p::GFP] animals per well were fed on these RNAi plates for two days at 20 degrees Celsius until L4/young adult stage. These animals were then screened for GFP expression.
Temperature20
Delivered_byBacterial_feeding
InhibitsPredicted_geneF45E1.6Inferred_automaticallyRNAi_primary
GeneWBGene00001945Inferred_automaticallyRNAi_primary
TranscriptF45E1.6.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
ReferenceWBPaper00041034
PhenotypeWBPhenotype:0001236RemarkThe zip-2(tm4248) mutation did not affect the RNAi-induced expression of the irg-1::GFP transgene
Remark(Table S1) his-71 RNAi. Authors state RNAi clones obtained from the Ahringer and Vidal laboratories, Ahringer clone used for curation.
MethodRNAi