WormBase Tree Display for RNAi: WBRNAi00097063
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| WBRNAi00097063 | Homol | Homol_homol | F53G12:RNAi | ||
|---|---|---|---|---|---|
| F56C11:RNAi | |||||
| Sequence_info | PCR_product | sjj_F56C11.1 | |||
| Experiment | Laboratory | GF | |||
| Date | 07 Nov 2011 00:00:00 | ||||
| Genotype | gst-4::GFP | ||||
| Treatment | RNAi was induced by feeding L1 worms through L4 stage with bacteria producing dsRNA to target genes. To induce bli-3 knockdown, the bacterial strain expressing bli-3 RNAi was diluted in a 1:30 ratio using the vector control. Worms were then exposed to the oxidative stress inducer Paraquat (100mM in M9) for 30 minutes. Worms were then transferred to normal NGM plates to recover for 4 hours before imaging GFP expression. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F53G12.3 | Inferred_automatically | RNAi_primary | |
| F56C11.1 | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00018771 | Inferred_automatically | RNAi_primary | ||
| WBGene00000253 | Inferred_automatically | RNAi_primary | |||
| Transcript | F56C11.1.1 | Inferred_automatically | RNAi_primary | ||
| F53G12.3.1 | Inferred_automatically | RNAi_primary | |||
| F56C11.1.2 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00040565 | ||||
| Phenotype_not_observed | WBPhenotype:0000306 | Remark | bli-3 RNAi did not affect paraquat-induced levels of gst-4::GFP transgene expression | ||
| Affected_by | Molecule | WBMol:00002747 | |||
| Remark | (Figure 5G, 5H) bli-3 RNAi | ||||
| Method | RNAi |
