WormBase Tree Display for RNAi: WBRNAi00101435

WBRNAi00101435 Homol: Homol_homol: CHROMOSOME_V:RNAi
      W10C8:RNAi
      CHROMOSOME_I:RNAi
  Sequence_info: DNA_text: tatcatgtgtctgctgcataaccgtcggatcaatctgctcgtcttcctcctcctcaacatcagattccgaatccgacgcgttcgcatccagcggactcctgcctgccgacgaggacgccagtggtgatggaacgtgcgtcgatgctcccgtcgtcgatcctagacgcatttgcatcaacatcgtatgcatcattgctgcttgctgctgctgtgtggtcagtgcaattccagcgtttgcctttggtgatgggctctccgagctactgctatacgcaccacttgtacctcgtccatcctgactgtcagttatatcggaaccgcccgaacgatcagttgcgtactcgcacttcttcttccgcttgcagaatttacaccacatttctgtgttgttaactccgaatctggctcggcacttcttctgatcgttgttctccgatgctggaaaaaaaatttttaatttattttaaagctggtaaattctgagcatgcgtattgtacaacatatttgacgcgaaaaatataaaaatatctcgtaacgaaaactacagtaattattaaaatgactaccgtagcgctgttaatttttccttccgctacagtagacatttatggggttattcatgtgatgtagaaaaatgaaaaaaacgcatccaatttttttgcatttgtggaaattttagaacatttttatgcatttttcgacattttttggaatttttcgacattttttggaatttttcggaatttttctacatttttgtgcattttttttttacattttttacacatttttagacatttttcgaaattttttgacatttttctgcatttttcgacagttcacttcacaagctgaaggcgaaaaaaatgccaaaaaaaatgccaaaaaatgcaaaaaaatgattgcatttttgtgcattttttctcatttttcaacattttttgtgcatttttctgcatttttcgattttttttttgcagttttcgacattggctgacagctcacttagcaagcggaaggcgaaaaaaatgccagaaaaatgtcaaaaaaatgcaaaaaatgattgcattttcccttttttttcaaaatttcctgcaaaattggaggttttagctaaaatttttgaaccggaaatttaccgaaaatttcagaatagcgaaattcgctttttttgggatattttttgaggcctaaacctcaaattttctgaaaaaaaaaatttcattgcggttcaaaatttccaacaaaaatggcataatgttagcggaaatctcaattcttggcagttttcccaggaaagttaggcaattttttttttttgaaaaattctcaaaattttgatcctaaaccgtaaaatttccgaaaaaaaaactcacgaatactcttatcccttcgtttcttcgtctttttcttattaaccgcataattttcccgcgccgaccactccggataccgttccttgtgtgtttccttatccttctttgccatttcaaaatatttcgcctgttcttccttcgacaaatcatgccacctctttccaagctctttattcaactctgcactctgtttctcattatttccaatctcttccagcagtgcttttcgattttccttcataaaccacatgaacgcgtttaatggcttcttcacatgatcatccttcttcgcttttccacctccttgagcccttcgatcgacattctgtggagccattgatgatggatgcatgtatggtggcattcccatctgattcattggatgaccggctcgcatgttcagtggagccatattctcaaagtgttgtttggcggctgccgcagcgattgctgctccatagacaggacttggcgggaacattggaaatgtggggctcattgctcccattggaaacatcaggcttggtgatcgtagtgctgctgcgtatggattctgaaaaaaaaatcgatttcaattttttcaaaaaactaaaaatcgaaaattttttgtttttcaattttccgatatttcgaacattttttttcgatttctcaaaaattagtttatcaatttaaatattttttctcatcaatttaaacaaaagtagatcgattttaaatttaaaaaaagctatttttaaaaattctaaaatgttcgttttaacgacacccttcatacgggtgtcggttttcccatttttcaaaaaaaaaatcgaaaacatttttttctttttttaaaatcaaattttccagaaaacatttctaaaaaaagtgcaaaaaagcaaatttcccactaatttttaacccaaaaaaatttttgtagcactctctctaactccctgagcggctggccccacctgtcctacagtacccccgagaagtttcttctcggaaactgttgtactgctctgctcaatatcctccaatcggcggccgctcgttaacaatatttgttttgcaagtctcgttcgcagctcattagagtatctgtgttatcactagttgctcacagagcacaaaacagtcacttgagatgtggaggggcttaagtcgaaatggaatagatacggatcagattttccgattgttgcctcaaagttttttttcaaatttatttatatataaatatttgtactctctgagagcaactttacattcaattgaaagtttcaaaaactcgaattattcgaaaatacctaaaagtaaaacggtaattggtctcaaagcgaccgactttttgaatctcaaaagaccgtgtgcccttaaggagtactgtagttttcaagatttgccgcacagctctgaaaggtacggtagtcattcagtttccacgaaatctactaaagagttcgaattcgtcagtgaaaactcaaatgtttgagtagattggtgtatttagtcacttgtctacagtacccctaacgagaaggagttgaaaaaaatgtagaggggataaaatatggcaattttcgcgccaaaactacagtacccgatctcgaagcgaagttaaattcaaacaaacttgcgcgcctttaagaagtactgtagttcctttagtttttgctacaaaaaccgaatggaaaatcgttcctaatttttcagaaaaccttggctcaacctgtgcatgtggtgtcaggctgccccttcacggtttgatctacaaaaaatgcgggaatttttcacccaaaaatatgtgacgtcaacaggttcttaaccatgcgaaatcagttgagagctctgcgtctcttctcccgcctattttgtagatctacgtagatcaagtcgaaatgagacactttgacaccacgtgtctgtgtacagtaccccgagaagataggcctagtaggtaggtacggtaatcattcagatccccgctagctctcaacaaattcaaaaaacaagtgtcaattctctacaggaagtggcaccaatcatgttccggaagcaattagagctctctcacacttgttctggtgtggggtcatcatcattttgtaatgaatgaatacgaacacacacacaccttttgaacctattgttttccgcgtctttttgtctgtagaaaaaaggtgtccaaacatgtgtgtgtggggggggggggggcccagaggtgtctaattgtctattgtatccgtgtctcgggtaccagcaaccataattaccaccaccatcgagaccccccaaagtgacgcaaaaggaaataatttcggctcttgacatgatgttcaacaattcaaaagaaatttttggtgttttttcgccaatagtttttcgagaaaaacaaattgccaaaaaacacgcaattttttgggagttaatgaggcttctccaacaaaaaagaagaaaaaactgttttcgaggaaatgcaacgagtttttggattcagaagataatagattcagtttttcggtgaaaattttgtagaatttctcacgaatttttggcttccctcataaaatgaaatggaagagtttttgccgaactaggtcattttggctcagtagatttacggcgcgttgcgtgtcgcgtcgcggctcgattttagttgtaaaactaaatgcacttgtccgtgtggagtacacgactatcccagtccggcgggcgattgtcaatggagcgcgaaaaattcaatgaggaaggccagaaccccatggcggaaagccaagaatttttggatttggaagacaataaattcagttttttagtggaaattttgtattatttctgctttttggggaagaaaattgtaggaagaataaaaaattaatacggttttttttctacaaaaatggagaaaagccctaaaaattcttgaaattgggtaaaaatttgaattttttttttttggaatttctgcttttttggggaagaaattgttggaaaaatgatttttaataggattttaaagcaattttttgcaaatttcgacgatttttgattcaaattgaagaataagctcaaaaatacacaattttccatatttttcaataaagctttcgattttagggtgttttttcgctttttttaaataatttttggtgtaaattcttaaattccaaaaatatagaggctctcgaaccctttaaaaattcaaaatacataatttttcccatttcttaggcaatttctgggcaaaatattgaaattgcaacttttaaaagtatttctaatgcctagactacataatttttctaataaaatagggggaattgtcataaaaaggattttaaagcgatttttcgtcgatttttgggggaaatcttgaaatttcaactttataaggtatttataacatattcgaggcctaatattcacctttgttttcagatttttcagtgaaaacttccaattaaagccgattttttgccaatttcggtaatttttcgttcagaagtatttaacttgttaactctaaaatacaattttaagtcgatttttgtcgattttcgccaatttttatgtgttttaattcaaattttttcaattttccaagtaaaaaatcaccataaatagaggcataaacatgggtaaagcagctgccgagtatccaggactgaagtgtgacattaaaccgggaaacgatggtgacattggttcgacttttggaaatgcactttttaacactgaaaatttgataattattcggttagaatggttaaaaaagagccaaatttttgattttcaaccgattttttggcaaatttcacaatttttagtcaaaattctttaaaatccaattttatagggttaaagatttcatttttccagattttttcatgaaaactccgattttaaagagatttttcggtaattttcacaatttttagttaaaattcttcaaaactcaattttatagggttaattatctcatttttccttcaaaatttcgatttttgaaagcgatttttcgcaaattttcacaattttcagttaaaaatattttaaaattcaacttttatagtgatatccgagttcaaaacctctcattttttagatttttcaattgaaaattcgattttaaagcgatttttcgtaaaattttaaaaggaatttctagcacctctaaaggcctgaaatacataattttttagatttttccattcacagatctcaaaaaaggtcaatttttggcgccaagttcaaatttctcagatcaattttgaatattatccaattcttaatcctttcgcacccctatacagtctattgagcacacacagacggaccaacttgttcaagtactcacgacggattagcacacaagataacggaccgagatagtatgggattcggagagacgcagatggtgttcgaacaccgttgcgtaacatccttttacgtcattatgtcattgcgtaaggcccgggaccatcaggtcaattcttaggggtgttttgaaattagcatggatttttttaatagaaaatagtgaaaaccggaaaaaaaattggttaaaaatgagtaaatttcatttataaagtgttagaaatactttaaaatataaaaaatggcgaaaaatcgctttaaaattggttgaaattcaaaataacactgaaaatactcaaatttgtcaaaaaaaaatattttagcgatttttaattaatttaaattcccgcgcttttgtctcgattttgaactccgcccataaaaccgtgccgcacttttgcactttttaatttcaacgtaaagtccaatttttgggttgtttttctagcttttaatgaatttttcggcgatttttaaaacctttttctccatttaaaaactgaaaaaaaacccccaaaatatggatttacgctgaatttgaaaaaaaaacaaagtgcggcacggttctatgggcggagtttagacaaaaaatgcgggaatttaaataaggtttttttttcaaatttaatgatttttttctctttttttgggatcagtcttagcatttaaagtttttatttaaattatttaaatttaaatttatatgtatttttttaatatttaatgtccctttttcctgcagaaatttagaaatttagaagaattttcaaatattttttttgtgtaataaagtcgaaaggcaattgaggtggtccataaaattttgtgtgatagctggccaccttcccctttcgatcaaatttattggaaaactcaccatcaatcgatggatttcgaccggcaccgtctaattctgcttccattacagcttcttttttatcatcggctaactgttgttctgacgtttcaccactaatcattggatcatcgtcagcatcctcatcccgacggaacaccttcacctcatcgccgagctcttcgtcggccatcatttttgt
  Experiment: Laboratory: JK
    Date: 23 Oct 2001 00:00:00
    Genotype: rde-1(ne219)/+; qIs56 [lag-2::GFP] (marker for the distal tip cell)
    Treatment: To circumvent pop-1 maternal effect lethality, double stranded pop-1 RNA was injected into a hermaphrodite worm homozygous for rde-1(ne219) (defective for RNAi). Injected animals were then mated with wild-type males to generate rde-1/+ cross-progeny, which are zygotically sensitive to RNAi. dsRNA was injected into unc-32; rde-1 animals at both 2.5 and 1 mg/ml. Injected animals were crossed with N2 males or males carrying GFP reporters.
    Delivered_by: Injection
  Inhibits: Predicted_gene: W10C8.2 Inferred_automatically: RNAi_primary
    Gene: WBGene00004077 Inferred_automatically: RNAi_primary
      WBGene00220126 Inferred_automatically: RNAi_primary
    Transcript: W10C8.2.1 Inferred_automatically: RNAi_primary
      W10C8.17 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00005116
  Phenotype: WBPhenotype:0000691 Remark: pop-1(RNAi) animals have no gonadal elongation and no bright lag-2::GFP expression in the gonad (unlike wild type). Somatic gonadal cells are aberrantly arranged around the gonadal periphery.
    WBPhenotype:0001278 Remark: pop-1(RNAi) animals have no gonadal elongation and no bright lag-2::GFP expression in the gonad (unlike wild type). Somatic gonadal cells are aberrantly arranged around the gonadal periphery.
    WBPhenotype:0001355 Remark: pop-1(RNAi) animals have no gonadal elongation and no bright lag-2::GFP expression in the gonad (unlike wild type). Somatic gonadal cells are aberrantly arranged around the gonadal periphery.
  Remark: (Figure 3C) zygotic pop-1 RNAi (see treatment details). Authors report using genomic sequence of gene as template DNA for dsRNA sequence. Entire genomic sequence used for curation.
  Method: RNAi