WormBase Tree Display for RNAi: WBRNAi00101798
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| WBRNAi00101798 | Homol | Homol_homol | T23D8:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_T23D8.9 | |||
| Experiment | Laboratory | LW | |||
| Date | 04 May 2009 00:00:00 | ||||
| Genotype | syIs187 [pes-10::7XTCF-mCherry-let-858(3'UTR); unc-119(+)] (a.k.a. 'POPTOP::mCherry' for POP-1/TCF Optimized Promoter) | ||||
| Treatment | To observe the effects of RNAi during postembryonic development, RNAi was performed by ingestion with a synchronous population of L1 larvae. L1 animals synchronized by standard methods were plated in triplicate on HT115(DE3) bacteria expressing dsRNA for genes of interest. Bacteria for ingestion were prepared using 4 mM IPTG to induce dsRNA production. RNAi-P was performed at 25 degrees Celsius and animals were scored for M lineage phenotypes or used for immunostaining 24-48 h after plating. | ||||
| Temperature | 25 | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | T23D8.9a | Inferred_automatically | RNAi_primary | |
| T23D8.9c | Inferred_automatically | RNAi_primary | |||
| T23D8.9b | Inferred_automatically | RNAi_primary | |||
| Gene | WBGene00006379 | Inferred_automatically | RNAi_primary | ||
| Transcript | T23D8.9b.1 | Inferred_automatically | RNAi_primary | ||
| T23D8.9c.1 | Inferred_automatically | RNAi_primary | |||
| T23D8.9a.1 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00033137 | ||||
| Phenotype | WBPhenotype:0001278 | Remark | sys-1(RNAi) results in dramatic decreases in expression of the POPTOP::mCherry transgene, indicative of loss of POP-1 transcriptional activator activity | ||
| Remark | (Figure 4W-Z) sys-1 RNAi-P (postembryonic RNAi) | ||||
| Method | RNAi |
