WormBase Tree Display for RNAi: WBRNAi00101916

WBRNAi00101916 Homol: Homol_homol: CC4:RNAi
  Sequence_info: PCR_product: sjj_CC4.2
  Experiment: Laboratory: IZ
    Date: 18 Jul 2014 00:00:00
    Genotype: L-AChR(gf) (ufIs6)
    Temperature: 20
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: CC4.2 Inferred_automatically: RNAi_primary
    Gene: WBGene00003753 Inferred_automatically: RNAi_primary
    Transcript: CC4.2.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Interaction: WBInteraction000523936
  Reference: WBPaper00045671
  Phenotype: WBPhenotype:0000643 Remark: This RNAi was able to suppress the locomotion phenotypes found in L-AChR(gf) animals. L-AChR(gf) enhances synaptic activation of muscles by increasing L-AChR function.
  Remark: The authors wanted to enhance synaptic activation of muscles by increasing L-AChR function, thus they introduced an amino acid change at a highly conserved position in the pore-lining M2 regions of cDNAs encoding the UNC-29, UNC-38 and LEV-1 subunits. They constructed a strain (ufIs6) stably expressing the unc-29(L/S), unc-38(V/S) and lev-1(L/S) cDNAs under control of the muscle-specific myo-3 promoter, and refer to this as L-AChR(gf).
  Method: RNAi