WormBase Tree Display for RNAi: WBRNAi00106271

WBRNAi00106271 Homol: Homol_homol: F46A9:RNAi
  Sequence_info: PCR_product: sjj_F46A9.4
  Experiment: Genotype: SKN-1::GFP
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: F46A9.4 Inferred_automatically: RNAi_primary
    Gene: WBGene00004808 Inferred_automatically: RNAi_primary
    Transcript: F46A9.4.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00044751
  Phenotype: WBPhenotype:0000306 Remark: "During normal development, SKN-1 shows transient expression in the EMS blastomere but rapidly diminishes upon EMS division so that no SKN-1 is detectable in later-stage EMS daughter cells (Bowerman et al., 1993; Page et al., 2007). We found that skr-1/2 was required for timely degradation of SKN-1 during EMS-to-MS transition. We used a SKN-1::GFP reporter to monitor SKN-1 expression (Page et al., 2007). In skr-1/2(RNAi), the initial level in the EMS blastomere was comparable to the wild-type. However, the reduction of protein level over time was significantly dampened and SKN-1 persisted for an additional cell cycle (Figure 5E). Importantly, we found that delayed degradation of SKN-1 caused EMS self-renewal."
      EQ_annotations: Anatomy_term: WBbt:0006876 PATO:0000460
  Remark: (Figure 5E) skr-1/2 RNAi. Authors state RNAi clones obtained from the Ahringer and Vidal laboratories, Ahringer clone used for curation. If Ahringer clone not available, Vidal laboratory clone used for curation.
  Method: RNAi