WormBase Tree Display for RNAi: WBRNAi00106292
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| WBRNAi00106292 | Homol | Homol_homol | R13G10:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_R13G10.1 | |||
| Experiment | Treatment | For DPY-27 RNAi, the bacterial strain from the Ahringer RNAi library containing dpy-27 RNAi plasmid and control plasmid were grown and induced in 100 ml LB media for 3 hours with 0.1 mM ITPG, concentrated 10-fold and seeded onto a 10 cm NGM plate supplemented with ampicillin, tetracycline and 1mM IPTG. Synchronized L1s were placed on seeded plates and collected at 24 hours (L3 larvae) or grown to gravid adults and embryos were collected by bleaching (mixed stage embryos). At collection time, all samples were washed 3 times in M9 buffer and stored in ten volumes of Trizol (Invitrogen) at -80C. 2-3 collections were pooled for one biological replicate. | |||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | R13G10.1 | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00001086 | Inferred_automatically | RNAi_primary | ||
| Transcript | R13G10.1.1 | Inferred_automatically | RNAi_primary | ||
| Species | Caenorhabditis elegans | ||||
| Reference | WBPaper00048953 | ||||
| Phenotype | WBPhenotype:0000718 | Remark | "In mixed stage embryos, dpy-27 RNAi mRNA-seq also showed derepression of X chromosomal genes. Derepression was slightly stronger for previously defined compensated genes, suggesting that posttranscriptional regulation contributes to the final mRNA levels in the DCC mutants." | ||
| Remark | dpy-27 RNAi | ||||
| Method | RNAi |
