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WormBase Tree Display for RNAi: WBRNAi00109123

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Name Class

WBRNAi00109123HomolHomol_homolY24D9A:RNAi
Sequence_infoPCR_productmv_G_YK1636
ExperimentStrainWBStrain00034068
GenotypezcIs13 [hsp-6p::GFP]
Treatment"RNAi bacteria were grown overnight in 96-well plates, while hsp-6p::gfp animals were synchronized at L1 larval stage. The next day, RNAi bacteria was induced with IPTG, resuspended in liquid NGM and added to reporter animals in 96-well plates. Animals were allowed to develop for 3 days and GFP was measured by fluorescent microscopy."
Delivered_byBacterial_feeding
InhibitsPredicted_geneY24D9A.8aInferred_automaticallyRNAi_primary
Y24D9A.8bInferred_automaticallyRNAi_primary
GeneWBGene00021286Inferred_automaticallyRNAi_primary
TranscriptY24D9A.8a.1Inferred_automaticallyRNAi_primary
Y24D9A.8b.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000537092
ReferenceWBPaper00045028
PhenotypeWBPhenotype:0001236RemarkRNAi resulted in induction of expression of GFP from the hsp-6 promoter
EQ_annotationsGO_termGO:0034514PATO:0000460
Remark(Figure 1b) Y24D9A.8 RNAi
MethodRNAi