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WormBase Tree Display for RNAi: WBRNAi00109139

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Name Class

WBRNAi00109139HomolHomol_homolE04A4:RNAi
Sequence_infoPCR_productmv_E04A4.5
ExperimentStrainWBStrain00034068
GenotypezcIs13 [hsp-6p::GFP]
Treatment"RNAi bacteria were grown overnight in 96-well plates, while hsp-6p::gfp animals were synchronized at L1 larval stage. The next day, RNAi bacteria was induced with IPTG, resuspended in liquid NGM and added to reporter animals in 96-well plates. Animals were allowed to develop for 3 days and GFP was measured by fluorescent microscopy."
Delivered_byBacterial_feeding
InhibitsPredicted_geneE04A4.5Inferred_automaticallyRNAi_primary
GeneWBGene00017119Inferred_automaticallyRNAi_primary
TranscriptE04A4.5.1Inferred_automaticallyRNAi_primary
SpeciesCaenorhabditis elegans
InteractionWBInteraction000537108
ReferenceWBPaper00045028
PhenotypeWBPhenotype:0001236RemarkRNAi resulted in induction of expression of GFP from the hsp-6 promoter
EQ_annotationsGO_termGO:0034514PATO:0000460
Remark(Figure 1b) E04A4.5 RNAi
MethodRNAi