WormBase Tree Display for RNAi: WBRNAi00110530
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| WBRNAi00110530 | Homol | Homol_homol | F49E7:RNAi | ||
|---|---|---|---|---|---|
| Sequence_info | PCR_product | sjj_F49E7.1 | |||
| Experiment | Genotype | GFP::LGG-1 | |||
| Treatment | Young adults (P0) were fed with RNAi bacteria, and the L3 progeny of the F1 generation were examined. | ||||
| Delivered_by | Bacterial_feeding | ||||
| Inhibits | Predicted_gene | F49E7.1a | Inferred_automatically | RNAi_primary | |
| Gene | WBGene00004377 | Inferred_automatically | RNAi_primary | ||
| Transcript | F49E7.1b.2 | Inferred_automatically | RNAi_primary | ||
| F49E7.1a.1 | Inferred_automatically | RNAi_primary | |||
| F49E7.1b.1 | Inferred_automatically | RNAi_primary | |||
| F49E7.1b.3 | Inferred_automatically | RNAi_primary | |||
| Species | Caenorhabditis elegans | ||||
| Interaction | WBInteraction000538725 | ||||
| Reference | WBPaper00038458 | ||||
| Phenotype | WBPhenotype:0000306 | Remark | Quantification of the number of GFP::LGG-1 puncta reveal that the average number of GFP::LGG-1 puncta was significantly increased, GFP::LGG-1 used as a reporter gene for autophagy as during autophagy GFP::LGG-1 forms fluorescent puncta. | ||
| WBPhenotype:0002282 | Remark | Quantification of the number of GFP::LGG-1 puncta reveal that the average number of GFP::LGG-1 puncta was significantly increased, GFP::LGG-1 used as a reporter gene for autophagy as during autophagy GFP::LGG-1 forms fluorescent puncta. | |||
| Remark | GFP::LGG-1 used as a reporter gene for autophagy as during autophagy GFP::LGG-1 forms fluorescent puncta. To minimize endogenous auto-fluorescence in adult animals, authors restricted their observation of GFP::LGG-1 positive foci to the hypodermal seam cells of L3 larvae. | ||||
| Method | RNAi |
