WormBase Tree Display for RNAi: WBRNAi00114765

WBRNAi00114765 Homol: Homol_homol: K05C4:RNAi
  Sequence_info: PCR_product: sjj_K05C4.6
  Experiment: Strain: WBStrain00000001
    Treatment: "For knockdown by feeding RNAi, bacterial cultures were plated on NGM plates supplemented with 25 μg/mL carbenicillin, 10 μg/mL tetracycline, and 1 mM IPTG, and incubated for 3 days at room temperature to induce double-stranded RNA. L4 worms at 20°C were fed bacteria for 48 hours and then singled to individual plates."
    Temperature: 20
    Delivered_by: Bacterial_feeding
  Inhibits: Predicted_gene: K05C4.6a Inferred_automatically: RNAi_primary
      K05C4.6b Inferred_automatically: RNAi_primary
    Gene: WBGene00001979 Inferred_automatically: RNAi_primary
    Transcript: K05C4.6a.1 Inferred_automatically: RNAi_primary
      K05C4.6b.1 Inferred_automatically: RNAi_primary
  Species: Caenorhabditis elegans
  Reference: WBPaper00060038
  Phenotype: WBPhenotype:0000072 Remark: "In wildtype animals, the positive control, hmp-2(RNAi), resulted in 17.9% of embryos that failed to hatch but this was not found to be significantly different to the L4440 control (p=0.068). It is important to note, however, that less than 1% of hatched hmp-2(RNAi) animals were normal larva; the vast majority were Hmp with severe body morphology defects."
      Penetrance: High
    WBPhenotype:0002608 Remark: "In wildtype animals, the positive control, hmp-2(RNAi), resulted in 17.9% of embryos that failed to hatch but this was not found to be significantly different to the L4440 control (p=0.068). It is important to note, however, that less than 1% of hatched hmp-2(RNAi) animals were normal larva; the vast majority were Hmp with severe body morphology defects."
      Penetrance: High
  Remark: hmp-2 (WBGene00001979) RNAi (Ahringer library clone)
  Method: RNAi