WormBase Tree Display for Strain: WBStrain00000127
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| WBStrain00000127 | Status | Live | ||
|---|---|---|---|---|
| Genotype | unc-119(ed3) III; niDf199 IV; armEx58. | |||
| Public_name | AGK233 | |||
| Contains | Gene | WBGene00006843 | ||
| Variation | WBVar00145093 | |||
| Rearrangement | niDf199 | |||
| Transgene | WBTransgene00015313 | |||
| Properties | Outcrossed | x0 | ||
| CGC_received | 16 Dec 2014 00:00:00 | |||
| Location | CGC | |||
| Remark | armEx58 [WRM0611aH08-Del8mer + unc-119(+)]. Pick non-Unc to maintain. This strain contains a transgenic array that expresses a derivative WRM0611aH08 fosmid. The WRM0611aH08 fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. This derivative fosmid construct lacks the upstream 8-mer motif (CTGTTTCA) next to 21U-3372. The expression of this individual 21U-RNA is lost in transgenic animals. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. | Inferred_automatically | From CGC strain data | |
| Made_by: Cecere & Mansisidore | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
