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WormBase Tree Display for Strain: WBStrain00000128

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Name Class

WBStrain00000128StatusLive
Genotypeunc-119(ed3) III; niDf199 IV; armEx53.
Public_nameAGK234
ContainsGeneWBGene00006843
VariationWBVar00145093
RearrangementniDf199
TransgeneWBTransgene00015312
PropertiesOutcrossedx0
CGC_received16 Dec 2014 00:00:00
LocationCGC
RemarkarmEx53 [WRM0611aH08 + unc-119(+)]. Pick non-Unc to maintain. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. This strain contains a transgenic array that expresses the WRM0611aH08 fosmid construct. This fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. Expression of this fosmid construct in JU258 worms restores the expression of the missing 21U-RNAs in the germline, as measured by RT-qPCR. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45.Inferred_automaticallyFrom CGC strain data
Made_by: Cecere & MansisidoreCGC_data_submission
SpeciesCaenorhabditis elegans