WormBase Tree Display for Strain: WBStrain00000128
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WBStrain00000128 | Status | Live | ||
---|---|---|---|---|
Genotype | unc-119(ed3) III; niDf199 IV; armEx53. | |||
Public_name | AGK234 | |||
Contains | Gene | WBGene00006843 | ||
Variation | WBVar00145093 | |||
Rearrangement | niDf199 | |||
Transgene | WBTransgene00015312 | |||
Properties | Outcrossed | x0 | ||
CGC_received | 16 Dec 2014 00:00:00 | |||
Location | CGC | |||
Remark | armEx53 [WRM0611aH08 + unc-119(+)]. Pick non-Unc to maintain. unc-119(ed3) was crossed into JU258, the niDf199IV deletion was confirmed by PCR, and these Unc worms were used for bombardment. This strain contains a transgenic array that expresses the WRM0611aH08 fosmid construct. This fosmid contains the niDF199 locus (around 4 kb) that is deleted in the natural C. elegans isolate strain JU258. JU258 worms lack specific 21U-RNAs normally present in N2 worms due to this deletion of the niDF199 locus. Expression of this fosmid construct in JU258 worms restores the expression of the missing 21U-RNAs in the germline, as measured by RT-qPCR. Reference: Cecere G, et al. Mol Cell. 2012 Sep 14;47(5):734-45. | Inferred_automatically | From CGC strain data | |
Made_by: Cecere & Mansisidore | CGC_data_submission | |||
Species | Caenorhabditis elegans |