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WormBase Tree Display for Strain: WBStrain00036648

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Name Class

WBStrain00036648StatusLive
Genotypegpb-1(ok1875)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC1506
ContainsGeneWBGene00001072
WBGene00001679
VariationWBVar00142982
WBVar00093071
RearrangementmIn1
mIn1[dpy-10(e128) mIs14(myo-2::GFP)]
TransgeneWBTransgene00000999
PropertiesOutcrossedx1
MutagenUV+TMP
CGC_received13 Mar 2007 00:00:00
LocationCGC
Made_byWBPerson20176
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F13D12.7. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok1875 homozygotes (early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: TTGATGTGGGGGTTTTAGGA. External right primer: ATCGCGTCGCTCACTAGAAT. Internal left primer: ATCGGGGAGAGAAAGAGAGC. Internal right primer: GGGGCACTATTGCATCATCT. Internal WT amplicon: 2980 bp. Deletion size: 1978 bp. Deletion left flank: GTAGAATACGATCGGGGAGAGAAAGAGAGC. Deletion right flank: ACGAGACACCCGCACGTTTCCCTCGCGTGT.Inferred_automaticallyFrom CGC strain data
Mutagen:UV/TMPCurator_confirmedWBPerson1983
SpeciesCaenorhabditis elegans