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WormBase Tree Display for Strain: WBStrain00036836

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Name Class

WBStrain00036836StatusLive
Genotypeszy-4(ok2324)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC1739
ContainsGeneWBGene00001072
WBGene00077732
VariationWBVar00142982
WBVar00093479
RearrangementmIn1
mIn1[dpy-10(e128) mIs14(myo-2::GFP)]
TransgeneWBTransgene00000999
PropertiesOutcrossedx1
MutagenEMS
CGC_received03 Jun 2009 00:00:00
LocationCGC
Made_byWBPerson20176
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
C30B5.1, C30B5.2. Homozygous sterile deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP ok2324 homozygotes (sterile, no eggs). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: AGGGGTACGGTCGAAAGTCT. External right primer: CCGACTGATCCTTATTCCGA. Internal left primer: AACACAGCGACGTCAGAATG. Internal right primer: GCAAGCATCATCGTCTTCAA. Internal WT amplicon: 2125 bp. Deletion size: 1066 bp. Deletion left flank: TCCAATTCAGATAGCAAACAGTGCATGCTT. Deletion right flank: GGTATCTTTAGTTTTATTTAAAATTTATGG.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans