WormBase Tree Display for Strain: WBStrain00037025
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WBStrain00037025 | Status | Live | ||
---|---|---|---|---|
Genotype | +/szT1[lon-2(e678)] I; mIs12 II; sec-3(ok2238)/szT1 X. | |||
Public_name | VC2003 | |||
Contains (4) | ||||
Properties | Outcrossed | x1 | ||
Mutagen | EMS | |||
CGC_received | 25 Sep 2014 00:00:00 | |||
Location | CGC | |||
Made_by | WBPerson20176 | |||
Remark | This strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use. | Paper_evidence | WBPaper00041807 | |
F52E4.7. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation, and homozygous for unlinked pharyngeal GFP insertion mIs12 (artifact of strain construction). Balanced lethal heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok2238 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain.External left primer: CAATCTTCGAGCCTGGGTAA. External right primer: TACCTTCCAGTCCAGATGCC. Internal left primer: TGAAATGGCGATTTTGATGA. Internal right primer: CATGATATGGCGATGCAAAG. Internal WT amplicon: 2918 bp. Deletion size: 1120 bp. Deletion left flank: TTTCTCCATACTACGTCCTCCGAGACTTGA. Deletion right flank: AATGAAACGATTTCCTCGTTGAGACGTTGC. | Inferred_automatically | From CGC strain data | ||
Species | Caenorhabditis elegans |