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WormBase Tree Display for Strain: WBStrain00037676

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Name Class

WBStrain00037676StatusLive
Genotypegpb-2(ok3691)/szT1 [lon-2(e678)] I; +/szT1 X.
Public_nameVC3111
ContainsGeneWBGene00001680
WBGene00003056
VariationWBVar00143398
WBVar00296655
RearrangementszT1
PropertiesOutcrossedx1
MutagenEMS
CGC_received30 Sep 2010 00:00:00
LocationCGC
Made_byWBPerson14471
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
F52A8.2. Apparent homozygous lethal deletion chromosome balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and ok3691 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: AATAATCAAGCCCAAATGCG. External right primer: CCAACAACTTGGGTTATGGC. Internal left primer: TTCCATCAGGAGAAGTTCGG. Internal right primer: ATCGCTTGCGGGTAAGATTT. Internal WT amplicon: 1318 bp. Deletion size: 393 bp. Deletion left flank: TTGTCACTTCTTCTCGAGGAGTACACTAGC. Deletion right flank: ACATGTTGAATCTCCACTTCCAGTTAAAAT.Inferred_automaticallyFrom CGC strain data
SpeciesCaenorhabditis elegans