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WormBase Tree Display for Strain: WBStrain00037712

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Name Class

WBStrain00037712StatusLive
GenotypeC17G10.2(gk3077)/mIn1 [mIs14 dpy-10(e128)] II.
Public_nameVC3188
ContainsGeneWBGene00001072
WBGene00015916
VariationWBVar00531878
WBVar00142982
RearrangementmIn1
TransgeneWBTransgene00000999
PropertiesOutcrossedx1
MutagenUV+TMP
CGC_received16 May 2011 00:00:00
LocationCGC
Made_byWBPerson20176
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
C17G10.2. Homozygous lethal deletion chromosome balanced by GFP- and dpy-10-marked inversion. Heterozygotes are WT with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP gk3077 homozygotes (embryonic or early larval arrest). Pick WT dim GFP and check for correct segregation of progeny to maintain. External left primer: AAGAATGCAAAACCTGAGAACAA. External right primer: TACAGCTGGATTTTCAACTGGAT. Internal left primer: ATACACCCGCTTTCCACAAG. Internal right primer: CCTCCAGCTTTCGATTTACG. Internal WT amplicon: 2029 bp. Deletion size: 368 bp. Deletion left flank: TTCGGTTACTCTTTGTTTTATATTTATTTT. Deletion right flank: TATTCAGTCTATGAAATACGATAAAGAAGA. Insertion Sequence: T.Inferred_automaticallyFrom CGC strain data
Mutagen:UV/TMPCurator_confirmedWBPerson1983
SpeciesCaenorhabditis elegans