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WormBase Tree Display for Strain: WBStrain00037731

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Name Class

WBStrain00037731StatusLive
Genotypebli-3(gk3069)/szT1 [lon-2(e678)] I; +/szT1 X.
Public_nameVC3226
ContainsGeneWBGene00000253
WBGene00003056
VariationWBVar00601110
WBVar00143398
RearrangementszT1
PropertiesOutcrossedx1
MutagenUV+TMP
CGC_received26 Oct 2011 00:00:00
LocationCGC
Made_byWBPerson14471
RemarkThis strain was provided by the C. elegans Reverse Genetics Core Facility at the University of British Columbia, which is part of the international C. elegans Gene Knockout Consortium, which should be acknowledged in any publications resulting from its use.Paper_evidenceWBPaper00041807
Apparent homozygous lethal deletion chromosome (gk3069 in F56C11.1) balanced by lon-2-marked translocation. Heterozygotes are WT, and segregate WT, Lon-2 males, arrested szT1 aneuploids, and gk3069 homozygotes (arrest stage/phenotype undetermined). Pick WT and check for correct segregation of progeny to maintain. External left primer: TGTGCAAATGAAGGAGCATC. External right primer: CTTCACACCGTTGGACATTG. Internal left primer: TCCACAACTGAACACTCCGA. Internal right primer: TTCAGGAAGCATTCTTTGGG. Internal WT amplicon: 1399 bp. Deletion size: 443 bp. Deletion left flank: CTGAACACTCCGATTTTGGATTGCTGCAAA. Deletion right flank: AGGAAATATACTTTACGGCAACGAACTCTG.Inferred_automaticallyFrom CGC strain data
Mutagen:UV/TMPCurator_confirmedWBPerson1983
SpeciesCaenorhabditis elegans