WormBase Tree Display for Strain: WBStrain00043478
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| WBStrain00043478 | Status | Live | ||
|---|---|---|---|---|
| Genotype | unc-119(ed3) III; kpIs100. | |||
| Public_name | IT1187 | |||
| Contains | Gene | WBGene00006843 | ||
| Variation | WBVar00145093 | |||
| Transgene | WBTransgene00024995 | |||
| Properties | Outcrossed | x0 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 28 Nov 2022 00:00:00 | |||
| Location | IT | |||
| CGC | ||||
| Made_by | WBPerson20892 | |||
| Remark | Generated based on CalTech XREF data | |||
| kpIs100 [pie-1p::Ub(G76V)::GFP::H2B::drp-1 3' UTR + unc-119(+)]. Expresses Ubiquitin::GFP fusion protein in the germ line. The last amino acid of ubiquitin is mutated to valine (G76V), which prevents the cleavage of ubiquitin from GFP by the deubiqutinating enzymes and renders the GFP constitutively targeted for degradation by the proteasome. The strain can be used to assay proteasome activity in the germ line. Reference: Kumar GA & Subramaniam K. Development. 2018 Mar 29;145(7):dev163949. PMID: 29540500 | Inferred_automatically | From CGC strain data | ||
| Species | Caenorhabditis elegans |
