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WormBase Tree Display for Strain: WBStrain00045186

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Name Class

WBStrain00045186StatusLive
Public_nameRS2333
PropertiesPhenotypeWBPhenotype:0000010Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Remark25 mM betaine delivered via the medium, almost every P. pacificus became paralyzed after four hours compared to ~30% of C. elegans.Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
ImageWBPicture0000014939Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004520Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
TreatmentNematodes were cultured on OP50 E. coli and NGM media at 20C. 20 J4 P. pacificus or L4 C. elegans were picked from a non-starved culture and transferred onto an unseeded plate or liquid buffer containing the drug compound at 20C for the duration of the experiment. 2.5 M Betaine hydrochloride solution was made with deionized nuclease-free water and stored in 4C. Betaine was top plated onto 35 mm NGM plates containing ~6 ml of agar by spreading 60 l of the stock solution and left to dry overnight.Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
WBPhenotype:0000845Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
RemarkWe found that 0.50 mM levamisole caused ~90% of C. elegans and P. pacificus to become paralyzed after one hour. Interestingly, P. pacificus reached 90% paralysis within ten minutes of treatment, while C. elegans took between 10 minutes to an hour to reach the same percentage of paralysis. Although the initial paralysis of P. pacificus occurred faster than C. elegans, over the course of 6 hours P. pacificus was able to recover faster than C. elegans.Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
ImageWBPicture0000014939Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00004019Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
TreatmentNematodes were cultured on OP50 E. coli and NGM media at 20C. 20 J4 P. pacificus or L4 C. elegans were picked from a non-starved culture and transferred onto an unseeded plate or liquid buffer containing the drug compound at 20C for the duration of the experiment. 0.10 M Levamisole stock solution was made with deionized nuclease-free water. Stock solutions were stored in -20C. Levamisole was added to NGM media without cholesterol before being poured: ~6 ml of agar into 35 mm plates.Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
WBPhenotype:0001611Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
RemarkWe found that while most worms in both nematode species were paralyzed within the first hour, C. elegans recovered faster than P. pacificus.Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
ImageWBPicture0000014939Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00001705Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Treatment10% Halothane solution was diluted in 100% ethanol in 1 ml tubes covered with aluminum foil under the fume hood. Twenty L4 or J4 worms were individually picked from seeded plates and transferred onto an unseeded 35 mm NGM plate. Under the fume hood with the lights off, 10 l of diluted halothane is added to a small piece of Whatman filter paper made by a standard office hole-puncher and placed on the lid. The dish was parafilmed immediately, covered with aluminum foil, and stored in 20C for the duration of the experiment.Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
WBPhenotype:0001856Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
RemarkWe found that in liquid swim assays with 0.051 mg/ml ivermectin, C. elegans became paralyzed faster than P. pacificus.Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
ImageWBPicture0000014939Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Affected_byMoleculeWBMol:00002786Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
Phenotype_assayControl_strainWBStrain00000001Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
TreatmentNematodes were cultured on OP50 E. coli and NGM media at 20C. 20 J4 P. pacificus or L4 C. elegans were picked from a non-starved culture and transferred onto an unseeded plate or liquid buffer containing the drug compound at 20C for the duration of the experiment. A 25 mg/ml ivermectin stock solution (28.57 mM) was diluted in 100% ethanol and stored in -20C. Twenty L4 or J4 worms were individually transferred into a 96-well flat-bottom tissue culture plates (Cellstar 655 180). Each well contained 100 l of 1:500 dilutions of ethanol or ivermectin in Nanopure water (58 M ivermectin).Paper_evidenceWBPaper00058964
Curator_confirmedWBPerson712
LocationRS
RemarkGenerated based on WC-CalTech XREF data
WBStrain mapped, WBPaper00061385 added based on AFP_Strain data.Curator_confirmedWBPerson1983
ReferenceWBPaper00058964
WBPaper00065793
SpeciesCaenorhabditis elegans