WormBase Tree Display for Strain: WBStrain00050636
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| WBStrain00050636 | Status | Live | ||
|---|---|---|---|---|
| Genotype | antIs30 II; unc-119(ed9) III. | |||
| Public_name | BRC546 | |||
| Contains | Gene | WBGene00006843 | ||
| Variation | WBVar00145096 | |||
| Transgene | WBTransgene00032857 | |||
| Properties | Outcrossed | x0 | ||
| Mutagen | MosSCI | |||
| CGC_received | 10 Mar 2021 00:00:00 | |||
| Location | CGC | |||
| Remark | antIs30 [attP-f + Cbr-unc-119(+) + glh-2p::phiC31 + rol-6(partial) + myo-2p::GFP + attP-r] II. antIs30 was inserted into ttTi5605 on LG II using MosSCI. GFP expression in pharynx is very weak (as it is single copy) and is easiest to see during the L1-L3 stages. This strain contains a phiC31 docking site and can be used for precise single-copy integration of transgenes via recombination mediated cassette exchange. The docking site contains inverted phiC31-attP sites flanking phiC31 integrase expressed from the glh-2 germline promoter. Integration constructs need to have inverted phiC31-attB sites that flank the intended sequence to be inserted. Reference: Yang FJ, et al. phiC31 integrase for recombination mediated single copy insertion and genome manipulation in C. elegans. Genetics 2021. | Inferred_automatically | From CGC strain data | |
| Made_by: Fang-Jung Yang | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
