WormBase Tree Display for Strain: WBStrain00051042
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| WBStrain00051042 | Status | Live | ||
|---|---|---|---|---|
| Genotype | rpa-1(iow117)/mIn1[mIs14 dpy-10(e128)] II. | |||
| Public_name | SSM596 | |||
| Contains | Gene | WBGene00001072 | ||
| WBGene00017546 | ||||
| Variation | WBVar00142982 | |||
| Rearrangement | mIn1 | |||
| Transgene | WBTransgene00000999 | |||
| Properties | Outcrossed | x1 | ||
| Mutagen | CRISPR_Cas9 | |||
| CGC_received | 01 Mar 2021 00:00:00 | |||
| Location | CGC | |||
| Remark | Crispr/Cas9-engineered indel in the 5 region of rpa-1. Larval-lethal mutation balanced by GFP- and dpy-10-marked inversion. Heterozygotes are wild-type with relatively dim pharyngeal GFP signal, and segregate WT dim GFP, Dpy bright GFP (mIn1 homozygotes), and non-GFP iow117 homozygotes (larval lethal). Pick wild-type dim GFP and check for correct segregation of progeny to maintain. iow117 was generated in mre-11::GFP background and outcrossed to N2. Reference: Hefel et al., Nucleic Acids Res. 2021 Jan 21;gkaa1293. doi: 10.1093/nar/gkaa1293. | Inferred_automatically | From CGC strain data | |
| Made_by: Adam Hefel | CGC_data_submission | |||
| Species | Caenorhabditis elegans |
