WormBase Tree Display for Strain: WBStrain00051718
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| WBStrain00051718 | Status | Live | |
|---|---|---|---|
| Genotype | wrdSi3 II; dpy-27(xoe41[dpy-27::AID::myc]) III; him-8(me4) IV. | ||
| Public_name | JEL1197 | ||
| Contains | Gene | WBGene00001086 | |
| WBGene00001867 | |||
| Variation | WBVar02157510 | ||
| WBVar00088864 | |||
| Properties | Outcrossed | x4 | |
| Mutagen | CRISPR_Cas9 | ||
| CGC_received | 10 Mar 2022 00:00:00 | ||
| Location | CGC | ||
| Remark | wrdSi3 [sun-1p::TIR1::F2A::mTagBFP2::AID*::NLS::tbb-2 3'UTR] (II:0.77). Endogenous dpy-27 locus tagged with AID element and myc to create a conditional allele using the auxin-inducible degron (AID). In absence of auxin, ~40% of worms are male due to him-8(me4) mutation. In the presence of auxin, ~100% of worms are male (hermaphrodite-lethal due to degradation of AID-tagged DPY-27). Reference: Li Q, et al. Inducible degradation of dosage compensation protein DPY-27 facilitates isolation of Caenorhabditis elegans males for molecular and biochemical analyses. bioRxiv 2022.01.27.478040; doi: https: | ||
| Made_by: Arshdeep Kaur | CGC_data_submission | ||
| Species | Caenorhabditis elegans |
