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WormBase Tree Display for Strain: WBStrain00051778

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Name Class

WBStrain00051778StatusLive
GenotypeieSi61 II; daf-2(hq363[daf-2::degron::mNeonGreen]) unc-119(ed3) III.
Public_nameMQD2374
ContainsGeneWBGene00000898
WBGene00006843
VariationWBVar02145361
WBVar00145093
PropertiesOutcrossedx0
MutagenCRISPR_Cas9
CGC_received19 Oct 2021 00:00:00
LocationCGC
RemarkieSi61 [ges-1p::TIR1::mRuby::unc-54 3' UTR + Cbr-unc-119(+)] II. Degron and mNeonGreen tag inserted at the 3' end of the endogenous daf-2 gene locus by CRISPR/Cas9 engineering. A single copy transgene was inserted into chromosome II (oxTi179) expressing modified Arabidopsis thaliana TIR1 tagged with mRuby in the intestine. This strain can be used for auxin-inducible degradation (AID) of DAF-2 protein in the intestine. Reference: Zhang Y, et al. BioRxiv. 2021 Aug 2. 2007.2031.454567. https:
Made_by: Yan-Ping Zhang, Wen-Hong ZhangCGC_data_submission
SpeciesCaenorhabditis elegans